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The septate junction (SJ) provides an occluding function for epithelial tissues in invertebrate organisms. This ability to seal the paracellular route between cells allows internal tissues to create unique compartments for organ function and endows the epidermis with a barrier function to restrict the passage of pathogens. Over the past twenty-five years, numerous investigators have identified more than 30 proteins that are required for the formation or maintenance of the SJs in Drosophila melanogaster, and have determined many of the steps involved in the biogenesis of the junction. Along the way, it has become clear that SJ proteins are also required for a number of developmental events that occur throughout the life of the organism. Many of these developmental events occur prior to the formation of the occluding junction, suggesting that SJ proteins possess non-occluding functions. In this review, we will describe the composition of SJs, taking note of which proteins are core components of the junction versus resident or accessory proteins, and the steps involved in the biogenesis of the junction. We will then elaborate on the functions that core SJ proteins likely play outside of their role in forming the occluding junction and describe studies that provide some cell biological perspectives that are beginning to provide mechanistic understanding of how these proteins function in developmental contexts. 

Abstract Aligning water supply with demand is a challenge, particularly in areas with large seasonal variation in precipitation and those dominated by winter precipitation. Climate change is expected to exacerbate this challenge, increasing the need for long‐term planning. Long‐term projections of water supply and demand that can aid planning are mostly published as agency reports, which are directly relevant to decision‐making but less likely to inform future research. We present 20‐year water supply and demand projections for the Columbia River, produced in partnership with the Washington State Dept. of Ecology. This effort includes integrated modeling of future surface water supply and agricultural demand by 2040 and analyses of future groundwater trends, residential demand, instream flow deficits, and curtailment. We found that shifting timing in water supply could leave many eastern Washington watersheds unable to meet late‐season out‐of‐stream demands. Increasing agricultural or residential demands in watersheds could exacerbate these late‐season vulnerabilities, and curtailments could become more common for rivers with federal or state instream flow rules. Groundwater trends are mostly declining, leaving watersheds more vulnerable to surface water supply or demand changes. Both our modeling framework and agency partnership can serve as an example for other long‐term efforts that aim to provide insights for water management in a changing climate elsewhere around the world.